Tanda offers a complete custom antibody production service covering every step of monoclonal and poly clonal development. Our capabilities include peptide antigen design (including phospho peptide), synthesis and carrier protein conjugation, immunization, serum collection or hybridoma fusion, and antibody purification. Many customization options are available, such as antibody conjugation, lyophilization, custom aliquoting, and antibody validation by Western blot, ELISA, or immunoprecipitation.

Option 1: Polyclonals raised against a peptide (Customer-provided peptide)

Production Time: 5 weeks

Description:

      1. Customer provides 1 mg non-conjugated or 2 mg KLH-conjugated (90% purity) peptide

      2. Immunize two rabbits (other species available)

      3. Perform test bleeds, titer tests

      4. Purify serum by protein A/G column

      5. Perform antigen affinity purification if requested

Deliverables: 10 mg purified antibody

Option 2: Polyclonals raised against a peptide (Tanda makes the peptide)

Production Time: 5 weeks

Description:

      1. Design, synthesize and conjugate the peptide antigen (15-20-mer, 5-10 mg)

      2. Immunize two rabbits (other species available)

      3. Perform test bleeds, titer tests

      4. Purify serum by protein A/G column

      5. Perform antigen affinity purification if requested

Deliverables: 10 mg purified antibody

Option 3: Polyclonals raised against a phospho-peptide

                  Production Time: 8-10 weeks

                  Description:

                       1. Design, synthesize and conjugate the phosphorylated peptide antigen (15-20-mer, 5-10 mg)

                       2. Immunize two rabbits (other species available)

                       3. Perform test bleeds, titer tests

                       4. Purify serum by protein A/G column

                       5. Perform antigen affinity purification if requested

             Deliverables: 10 mg purified antibody

Option 4: Polyclonals raised against a protein

                  Production Time: 5-7 weeks

                  Description:

                       1. Customer provides 5 mg of protein antigen

                       2. Immunize two rabbits (other species available)

                       3. Perform test bleeds, titer tests

                       4. Purify serum by protein A/G column

                       5. Perform antigen affinity purification if requested

                  Deliverables: 10 mg purified antibody