Peptide Labeling
Tanda can help you design and create the best labeled peptide or protein for your experimental application. And can help you determine the best label or modification based on your peptide sequence and experimental conditions. All labeled peptides come with HPLC analysis standard to ensure purity and quality of the product.
Tanda's most popular labeling and modifications can be found below. Of course, we can also use more specialized labels upon request.
Fluorescent Dye Labeling: Tanda can deliver your labeled peptides in about 2 working days, with over 17 fluors such as FITC, FAM, APC, PE, Cy3, Cy5, Cy7, and more.
Biotin and Enzyme Labeling: Biotin and enzymes such as HRP and AP are widely used labels, which can be incorporated into peptides or labeled peptides at many locations. Usually, they are incorporated into the N-terminal or Lys side chain. Tanda can provide Biotin or Enzyme-labeled peptides according to your requirements.
Custom Labeling: Based on the specific requirements of our clients, we can customize the fluorescence labeling process. This encompasses the selection of the appropriate dye, optimization of labeling conditions to maintain the biological function of the molecule and increase its stability.
Peptide-Carraier Protein Conjugation
Peptides must be conjugated to a carrier protein to induce the formation of antibodies to elicit an immune response. The most common method for conjugating peptides to proteins is based on thiolmaleimide chemistry - a highly specific and robust reaction. Tanda offers a variety of carrier proteins such as KLH, BSA, OVA, etc. for custom peptide conjugates, and assist in selecting the best carrier to ensure optimal antibody production.
KLH: KLH is the most common carrier protein. It is more immunogenic than other conjugated proteins.
BSA: BSA is the most stable and soluble albumin, containing 59 lysines, of which 30-35 can bind. It is a commonly used carrier protein for weakly antigenic compounds.
OVA: OVA is a protein isolated from chicken eggs. It is often used as a control carrier protein to verify that the antibody is directed against the target peptide and not the carrier protein.