We provide a custom acetyl-specific antibody production service. Typically such a project is a rabbit polyclonal anti-peptide antibody, which is what is outlined in the below. However, should your requirements differ from this they can be accommodated. Please contact us for a free consultation in order to determine your requirements as well as project feasibility and estimated cost.

The acetyl-specific service package includes;

        1. Design and synthesis of acetylated immunisation peptide (10 mg, high purity) and a non-acetyl control peptide.
        2. Conjugation of the immunisation peptide to KLH
        3. Immunisation of two rabbits using a 12 week standard protocol.
        4. Collection of pre-immune serum and three immune serum samples.
        5. ELISA testing of first serum sample against the acetyl and non-acetyl peptides.
        6. Acetyl specific affinity purification using a total of 20 ml of crude serum.

Additional services can be added to this package. Some examples include;
        1. Antibody labelling, such as biotin or FITC.

The projected time line for your custom acetyl-specific polyclonal rabbit antibody project is as follows;

        1. 5 weeks - Peptide design, production and conjugation
        2. 6 weeks - First serum sample after primary immunisation
        3. 3 weeks - Additional serum sample.
        4. 2 weeks - Acetyl-specific affinity purification.

Additonal information
        1. Antibodies raised against synthetic peptides