Co-immunoprecipitation (Co-IP) is one of the most commonly used methods for determining protein-protein interactions. It relies on the ability of an antibody to stably and specifically bind complexes containing a bait protein. The antibody provides a means of immobilizing these complexes on a solid matrix. In this way, it is possible to detect the unknown members of proteins or ligands that are bound to the specific protein in natural condition.

Tanda can perform your Co-IP experiments using the latest rapid liquid-phase reaction kinetics and magnetic (or resin) separation, which insure proper antibody orientation for optimal antigen binding and save on antibody usage.

Detection Options:

    1. 1D/2D Electrophoresis - determination the molecular weight and isoelectric point of immunoprecipitated proteins
    2. 2D Blue Native / SDS-PAGE - analysis of the subunits of membrane protein complexes
    3. Western Blot analysis - detection of specific proteins in immunoprecipitated sample
    4. Shotgun proteomics - identification and quantification of peptides from digested proteins using tandem mass spectrometry
    5. LC-MS-MS – separation, identification and quantification substances in immunoprecipitated mixtures

Because of the biochemical diversity of protein-protein interactions, it is not possible to describe a single set of conditions that will work for every immunoprecipitation experiment. Instead, our technical team can provide both practical starting conditions and potential modifications to the procedure so that best conditions for your Co-immunoprecipitations are optimized.

Welcome to discuss your Co-immunoprecipitations experiment with our technical team. We will help you to develop the optimal Co-immunoprecipitations procedure to reach the best results.

1. High Sensitivity and Specificity: Utilizing high-quality antigens, antibodies, and enzyme conjugates to ensure high sensitivity and specificity, enabling accurate detection of low-concentration targets while effectively avoiding interference from non-specific reactions, guaranteeing the accuracy and reliability of test results.

2. Broad Detection Range: Capable of detecting various types of target substances, including proteins, peptides, hormones, antibodies, antigens, and pathogens, covering a wide range of applications in biomedical research and clinical diagnostics. Additionally, the wide detection range meets the testing needs of target substances at different concentration levels.

3. Strict Quality Control: Every step from sample collection to result reporting strictly follows standardized operating procedures and a quality control system to ensure the reproducibility and stability of experimental results. Regular calibration and maintenance of instruments, along with the use of standards and quality control materials, are implemented to monitor and validate the experimental process.

4. Customed Service Solutions: Tailored experimental protocols are designed based on clients' specific research needs and sample characteristics, including sample processing methods, detection method selection, and antibody screening, ensuring that the experimental results best meet clients' research objectives.

5. Professional Technical Team: A highly experienced and skilled technical team with strong backgrounds in immunology and molecular biology, proficient in all aspects of ELISA technology, provides comprehensive technical support and consulting services—from experimental design, sample processing, and assay execution to result analysis and interpretation.

6. Fast and Efficient Service: With optimized workflows and an efficient team, testing projects can be completed in a short time frame, delivering results promptly to meet clients' deadlines.