We provide a custom methyl-specific antibody production service. Typically such a project is a rabbit polyclonal anti-peptide antibody, which is what is outlined in the below. However, should your requirements differ from this they can be accommodated. Please contact us for a free consultation in order to determine your requirements as well as project feasibility and estimated cost.

The methyl-specific service package includes;

          1. Design and synthesis of methylated immunisation peptide (10 mg, high purity) and a non-methyl control peptide.

          2. Conjugation of the immunisation peptide to KLH

          3. Immunisation of two rabbits using a 12 week standard protocol.

          4. Collection of pre-immune serum and three immune serum samples.

          5. ELISA testing of first serum sample against the methylated and non-methyl peptides.

          6. Methyl-specific affinity purification using a total of 20 ml of crude serum.

Additional services can be added to this package. Some examples include;

          1. Antibody labelling, such as biotin or FITC.

The projected time line for your custom methyl-specific polyclonal rabbit antibody project is as follows;

          1. 5 weeks - Peptide design, production and conjugation

          2. 6 weeks - First serum sample after primary immunisation

          3. 3 weeks - Additional serum sample.

          4. 2 weeks - Methyl-specific affinity purification.

Addtional information:

          1. Designing synthetic peptides as antigens for antibody production.