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Fluorescent Labeling Dye
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FITC-Dextran (MW 40000)
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FITC-Dextran (MW 40000)
TD-128868D
　  
生物活性 　 FITC-Dextran (MW 40000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 40000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 40000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption. 　
体外研究
(In Vitro)		 　 Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

细胞标记:
适用于正在发生凋亡的 HeLa 细胞和人外周血单个核细胞 (PBMC) (活的 HeLa 和 PBMC 不被 FITC-Dextran 染色)。
1. 43.5°C 孵育 1 h，37°C 孵育 8 h，诱导细胞凋亡。
2. 将细胞悬浮在 100 μL 的培养基中，在 Q-prep 管与 10 μL 的 propidium iodide (PI)，10 μL 的 FITC-Dextran (MW 40000) 混合，PI 和 FITC-Dextran (MW 40000) 的最终浓度分别为 7.5 μM 和 1.13 μM。
3. 将细胞在室温黑暗条件下培养 25 分钟。
4. 取 3 mL 培养基的标记细胞，500 g 离心 10 分钟。
5. 取离心细胞，加入 1 mL 培养基，用流式细胞术或荧光显微镜分析 (PI: Ex=500 nm, Em=600 nm; FITC-Dextran (MW 40000): Ex=495 nm, Em=525 nm)。


细胞旁渗透性检测
1. 在 transwell 室的基础培养基中加入 FITC-Dextran (0.1 mg/mL)。
2. 在 15 分钟后从 transwell 插入器中收集培养基。
3. 测量荧光信号 (Ex=485 nm, Em=538 nm)。
4. 根据荧光强度计算 FITC-Dextran 浓度。
5. 计算渗透率。

Tanda has not independently confirmed the accuracy of these methods. They are for reference only.		 　
体内研究
(In Vitro)		 　 Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

肠屏障功能测定
1. 小鼠饥饿处理 4 小时。
2. FITC-Dextran MW 40000 灌胃小鼠 (0.6 mg/g)。
3. 在 4 h 内测量荧光强度 (Ex nm/Em 520 nm)。
Tanda has not independently confirmed the accuracy of these methods. They are for reference only.		 　
分子量 　 40000(Average) 　
CAS 号 　 60842-46-8 　
性状 　 固体 　
颜色 　 Yellow to orange 　
Emission (Em) 　 525 　
Excitation (Ex) 　 488 　
运输条件 　 Room temperature in continental US; may vary elsewhere. 　
储存方式 　 4°C, protect from light 　
　 * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) 　
溶解性数据 细胞实验： 
H2O 中的溶解度 : ≥ 5.88 mg/mL (6.88 mM)