Product Name |
COX IV mouse monoclonal antibody |
Cat.# |
TD-BYX-0040 |
Package |
25 μl/100 μl/200 μl |
Storage |
PBS with 0.1% sodium azide and 50% glycerol, pH 7.2 |
Product Imformation |
Description |
Anti-COX IV mouse monoclonal antibody |
Applications |
WB |
Immunogen |
Fusion protein of human COX4 |
Reactivity |
Human |
Host species |
Mouse |
Ig class |
IgG1 |
Purity |
Protein A/G Affinity Purification |
Target information |
Symbol |
COX4 |
Full name |
cytochrome c oxidase subunit 4 |
Synonyms |
COX4; COXIV; COX4-1; COXIV-1; COX IV |
Target Background |
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. |
Western blotting |
Predicted band size |
20 kDa |
Positive control |
HUVEC cell, HepG2 cell, Human heart tissue, Jurkat cell, 293T cell lysates |
Recommended dilution |
5000-20000 |
Gel |
12%SDS-PAGE |
Lysate |
40 μg |
Lane 1-5 |
HUVEC cell, HepG2 cell, Human heart tissue, Jurkat cell, 293T cell lysates |
Primary antibody |
D191070(COX4I1 Antibody) at dilution 1/12000 |
Secondary antibody |
Goat anti mouse IgG at 1/5000 dilution |
Exposure time |
1 minute |
Product Image |
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