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Point Mutation Antibodies
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Anti-IDH1 (R132H) Mouse Monoclonal Antibody
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TD-260811
TD-260812
  • Catalog： TD-26081
  • Clonality： mAb
  • Application： ELISA, WB, IHC, IF
  • Synonyms：  ​​​​​​​IDH1(R132H)
  • Specification：
    100ul
  • Prices：
    ¥4800
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Anti-IDH1 (R132H) Mouse Monoclonal Antibody

Cat. #:   TD-26081
Gene Symbol:  IDH1
Description:   Anti-IDH1(R132H) Mouse Monoclonal Antibody
Background:   Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
Immunogen:   Recombinant protein of human IDH1 which includes the mutation of R132H.
Applications:   ELISA, WB, IHC, IF
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IF: 1:50-1:100
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-IDH1(R132H) antibody recognizes IDH1(R132H) of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

TD-260811.jpg

 

Immunofluorescence:  

TD-260812.jpg

 

 

Western blot analysis of recombinant IDH1(R132H) and wild type proteins.

 

 

 

 

Immunofluorescence of cells expressing IDH1 proteins with anti-IDH1(R132H) antibody.

HEK293T cells were transfected with pCDNA3-GFP-IDH1 (WT) plasmid (left column) or pCDNA3-GFP-IDH1(R132H) plasmid (right column), then fixed and stained with Anti-IDH1(R132H) monoclonal antibody (Cat. # TD-26081).