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Point Mutation Antibody
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Anti-EGFR(G719A) Mouse Monoclonal Antibody
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TD-261151
TD-261152
  • Catalog： TD-26115
  • Clonality： mAb
  • Application： ELISA, WB, IF, IHC
  • Synonyms： EGFR(G719A)
  • Specification：
    100ul
  • Prices：
    ¥4800
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Anti-EGFR(G719A) Mouse Monoclonal Antibody

 

Cat. #:  TD-26115
Gene Symbol:  EGFR/HER1/ERBB1
Description:   Anti-EGFR(G719A) Mouse Monoclonal Antibody
Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of G719A, human origin.
Tested Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   ELISA: 1:1000-1:5000、WB: 1:500-1:1000、IHC: 1:50-1:100
Concentration:    1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR(G719A), but not wild type EGFR of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing.
Western blot:  

TD-261151.jpg

 
Immunofluorescence:  
 

TD-261152.jpg

 

 

 

 

Western blot analysis of recombinant EGFR(G719A) and wild type proteins.

Purified His-tagged EGFR(G719A) protein (aa 702-987) and wild type protein (aa 702-987) were blotted with anti- EGFR(G719A) mouse antibody (cat. # TD-26115).

 

 

 

 

 

 

 

Immunofluorescence of cells expressing EGFR proteins with anti-EGFR(G719A) antibody.

HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(G719A) plasmid (right column), then fixed and stained with anti-EGFR (G719A) monoclonal antibody (cat. # TD-26115).