All Products
  • Antibody
  • Kit
  • Peptide
  • Probe
  • Protein
  • Biochemical Reagent
  • Tissue Microarray
CH
ASGR1/ TL1A/ RSV/ TREM2/ Monkeypox virus/ BG4/ A29L/ IdeZ/ IdeS/ CD47/ claudin 18.2/ H3L/ SARS/ DNA/
Point Mutation Antibody
Path：Home > Product > Antibody > Point Mutation Antibody
Product
Product
Anti-EGFR (G863D) Mouse Monoclonal Antibody
All
TD-261191
TD-261192
  • Catalog： TD-26119
  • Clonality： mAb
  • Application： ELISA, WB, IF, IHC
  • Synonyms： EGFR(G863D)
  • Specification：
    100ul
  • Prices：
    ¥4800
Customized service consulting
Information
FAQ
Experimental program
Related Products
Bibliography

Anti-EGFR (G863D) Mouse Monoclonal Antibody

 

Cat. #:   TD-26119
Gene Symbol:  EGFR/HER1/ERBB1
Description:   Anti-EGFR(G863D) Mouse Monoclonal Antibody
Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of G863D, human origin.
Tested Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   ELISA: 1:1000-1:5000、WB: 1:1000-1:5000、IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Preservative: NoA
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR(G863D), but not wild type EGFR from vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing.

Western blot:  

TD-261191.webp

 
Immunofluorescence:  

TD-261192.webp

 

 

 

 

Western blot analysis of recombinant EGFR(G863D) and wild type proteins.

 

 

 

 

 

 

 

Immunofluorescence of cells expressing EGFR proteins with anti-EGFR(G863D) antibody.

HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(G863D) plasmid (right column), then fixed and stained with anti-EGFR(G863D) monoclonal antibody (Cat. #TD-26119).