| Cat. #: TD-26138 |
| Size: 100 µL |
| Gene Symbol: PIK3CA |
| Description: Anti-PIK3CA(Q546K) Mouse Monoclonal Antibody |
| Background: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform. Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Belongs to the PI3/PI4-kinase family. Located to cytosol, lamellipodium and phosphatidylinositol 3-kinase complex. |
| Immunogen: A synthetic peptide from the internal region of PIK3CA(Q546K) of human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 |
| Concentration: 0.2 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: recognizes Q546K mutant, but not wild type PIK3CA of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
|
Western blot:
|
Western blot analysis of PIK3CA wild type and Q546K proteins. Equal amount of purified PIK3CA(Q546K)(amino acids 461-640) and corresponding wild type proteins were loaded and blotted with anti-PIK3CA(Q546K) mouse monoclonal antibody(Cat. # TD-26138). |