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Point Mutation Antibodies
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Anti-IDH2 (R172K) Mouse Monoclonal Antibody
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TD-261631
TD-261632
TD-261633
  • Catalog： TD-26163
  • Clonality： mAb
  • Application： ELISA, WB, IHC
  • Synonyms： IDH2(R172K)
  • Specification：
    100ul
  • Prices：
    ¥4800
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Anti-IDH2 (R172K) Mouse Monoclonal Antibody

Cat. #:   TD-26163
Gene Symbol:  IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH
Description:   Anti-IDH2(R172K) Mouse Monoclonal Antibody
Background:   Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172K IDH2 mutations do exist in diffusely infiltrative gliomas.
Immunogen:   A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   ELISA: 1:1000-1:5000、WB: 1:100-1:1000、IHC: 1:50-1:100、IHC: 1:10-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  recognizes R172K mutant, but not wild type IDH2 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

TD-261631.jpg

Western blot analysis of recombinant IDH2(R172K) and wild type proteins.

Purified His-tagged IDH2(R172K) (lane 2) and wild type protein (lane 1) were blotted with Anti-IDH2(R172K) monoclonal antibody (Cat. #TD-26163).

Immunofluorescence:  

TD-261632.png

 

TD-261633.png
Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172K) antibody.

HEK293T cells were transfected with pCDNA3-GFP- IDH2(R172K) plasmid , pCDNA3-GFP- IDH2 (WT) plasmid, pCDNA3-GFP- IDH2 (R172H) plasmid , pCDNA3-GFP- IDH2 (R172W) plasmid, pCDNA3-GFP- IDH2 (R172G) plasmid,pCDNA3-GFP- IDH2 (R172M) plasmid or pCDNA3-GFP- IDH2 (R172S) plasmid, then fixed and stained with anti- IDH2(R172K) monoclonal antibody (Cat. #TD-26163).