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Point Mutation Antibody
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Anti-IDH2 (R140Q) Mouse Monoclonal Antibody
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TD-261651
TD-261652
  • Catalog： TD-26165
  • Clonality： mAb
  • Application： ELISA, WB, IF, IHC
  • Synonyms： IDH2(R140Q)
  • Specification：
    100ul
  • Prices：
    ¥4800
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Anti-IDH2 (R140Q) Mouse Monoclonal Antibody

 

Cat. #::  TD-26165
Gene Symbol:  IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH
Description:   Anti-IDH2(R140Q) Mouse Monoclonal Antibody
Background:   Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
Immunogen:   A synthetic peptide from the internal region of IDH2 which includes the mutation of R140Q, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   ELISA: 1:1000-1:5000、WB: 1:500-1:1000、IF: 1:50-1:100、IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes R140Q mutant, but not wild type IDH2 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

TD-261651.jpg

 Western blot analysis of recombinant IDH2(R140Q) and wild type proteins.

Purified His-tagged IDH2(R140Q) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with anti IDH2(R140Q) monoclonal antibody (Cat. #TD-26165).

Immunofluorescence:  

TD-261652.jpg
Immunofluorescence of cells expressing IDH2 proteins with anti IDH2(R140Q) antibody.

HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R140Q) plasmid (right column), then fixed and stained with anti-IDH2(R140Q) monoclonal antibody (Cat. #TD-26165).