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Point Mutation Antibody
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Anti-β-catenin (E53K) Mouse Monoclonal Antibody
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TD-261671
TD-261672
  • Catalog： TD-26167
  • Clonality： mAb
  • Application： ELISA, WB, IF, IHC
  • Synonyms： β-Catenin(E53K)
  • Specification：
    100ul
  • Prices：
    ¥4800
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Anti-β-catenin (E53K) Mouse Monoclonal Antibody

 

Cat. #:  TD-26167
Gene Symbol:  CTNNB1, CTNNB
Description:   Anti-β-catenin(E53K) Mouse Monoclonal Antibody
Background:   Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.
Immunogen:   A synthetic peptide from the internal region of β-catenin which includes the mutation of E53K, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:    ELISA: 1:1000-1:5000 、WB: 1:500-1:2000 、IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:   Recognizes β-catenin(E53K), but not wild type β-catenin protein from vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Western blot:  

TD-261671.jpg

 
 Immunofluorescence:  

TD-261672.jpg

 

Western blot analysis of recombinant β-catenin(E53K) and wild type proteins. Purified His-tagged β-catenin(E53K) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-β-catenin(E53K) mouse monoclonal antibody (Cat. #TD-26167).   

 

 

 

 

 

 

 

Immunofluorescence of cells expressing β-catenin proteins with anti-β-catenin(E53K) antibody. HEK293T cells were transfected with pCDNA3-GFP- β-catenin (WT) plasmid (left column) or pCDNA3-GFP-β-catenin(E53K) plasmid (right column), then fixed and stained with Anti-β-catenin (E53K) monoclonal antibody (Cat. #TD-26167).