All Products
  • Antibody
  • Kit
  • Peptide
  • Probe
  • Protein
  • Biochemical Reagent
  • Tissue Microarray
CH
ASGR1/ TL1A/ RSV/ TREM2/ Monkeypox virus/ BG4/ A29L/ IdeZ/ IdeS/ CD47/ claudin 18.2/ H3L/ SARS/ DNA/
Point Mutation Antibody
Path：Home > Product > Antibody > Point Mutation Antibody
Product
Product
Anti-β-catenin (G38D) Mouse Monoclonal Antibody
All
TD-261691
TD-261692
  • Catalog： TD-26169
  • Clonality： mAb
  • Application： ELISA, WB, IF, IHC
  • Synonyms： β-Catenin(G38D)
  • Specification：
    100ul
  • Prices：
    ¥4800
Customized service consulting
Information
FAQ
Experimental program
Related Products
Bibliography

Anti-β-catenin (G38D) Mouse Monoclonal Antibody

 

Cat. #:  TD-26169
Gene Symbol:  CTNNB1, CTNNB
Description:   Anti-β-catenin(G38D) Mouse Monoclonal Antibody
Background:   Beta-catenin(or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.
Immunogen:   A synthetic peptide from the internal region of β-catenin which includes the mutation of G38D, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:50-100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:    Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes β-catenin(G38D), but not wild type β-catenin protein from vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Western blot:  

TD-261691.jpg

 
Immunofluorescence:   

TD-261692.jpg

 

Western blot analysis of recombinant β-catenin(G38D) and wild type proteins. Purified His-tagged β-catenin(G38D) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-β-catenin(G38D) mouse monoclonal antibody (Cat. #TD-26169).    

 

 

 

 

 

Immunofluorescence of cells expressing β-catenin proteins with anti-β-catenin(G38D) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTNNB1 (WT) plasmid (left column) or pCDNA3-GFP-CTNNB1(G38D) plasmid (right column), then fixed and stained with anti-β-catenin(G38D) monoclonal antibody (Cat. #TD-26169).