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Point Mutation Antibody
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Anti-EGFR (vIII) Mouse Monoclonal Antibody
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TD-261761
TD-261762
  • Catalog： TD-26176
  • Clonality： mAb
  • Application： ELISA, WB, IF, IHC
  • Synonyms： EGFR(vIII)
  • Specification：
    100ul
  • Prices：
    ¥4800
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Anti-EGFR (vIII) Mouse Monoclonal Antibody

 

Cat. #:   TD-26176
Gene Symbol:  EGFR/HER1/ERBB1
Description:   Anti-EGFR(vIII) Mouse Monoclonal Antibody
Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (NSCLC).
Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of vIII, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:  ELISA: 1:1000-1:5000、WB: 1:1000-1:5000、IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR(vIII), but not wild type EGFR from vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

TD-261761.webp

  
Western blot analysis of recombinant EGFR(vIII) and wild type proteins.

Purified His-tagged EGFR(vIII) protein (partial, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-EGFR(vIII) mouse monoclonal antibody (Cat. # TD-26176).

Immunofluorescence:  

TD-261762.webp

 Immunofluorescence of cells expressing EGFR proteins with anti-EGFR(vIII) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(vIII) plasmid (right column), then fixed and stained with anti-EGFR(vIII) monoclonal antibody (Cat. #TD-26176).