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Point Mutation Antibody
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Anti-B-Raf (V600D) Mouse Monoclonal Antibody
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TD-261841
TD-261842
  • Catalog： TD-26184
  • Clonality： mAb
  • Application： ELISA, WB, IF, IHC
  • Synonyms： BRAF(V600D)
  • Specification：
    100ul
  • Prices：
    ¥4800
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Anti-B-Raf (V600D) Mouse Monoclonal Antibody

 

Cat. #:   TD-26184
Gene Symbol:  BRAF, BRAF1, RAFB1
Description:   Anti-BRaf(V600D) Mouse Monoclonal Antibody
Background:   B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
Immunogen:   A synthetic peptide from the internal region of BRaf(V600D), human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:    ELISA 1:1000-1:5000 WB 1:500-1:2000 IF 1:100-1:200 IHC 1:100-1:200
Concentration:    0.5 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes B-Raf V600D, but not wild type B-Raf proteins from vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:

TD-261841.jpg

  Western blot analysis of recombinant B-raf proteins. Purified His-tagged BRaf(V600D) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-BRaf(V600D) monoclonal antibody (Cat. # TD-26184). 

Immunofluorescence:  

TD-261842.jpg

 Immunofluorescence of cells expressing B-Raf proteins with Anti-BRaf(V600D) antibody. HEK293T cells were transfected with pCDNA3-GFP-B-Raf (WT) plasmid (left column) or pCDNA3-GFP-BRaf(V600D) plasmid (right column), then fixed and stained with Anti-BRaf(V600D) monoclonal antibody (Cat. #TD-26184).