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Point Mutation Antibodies
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Anti-HRAS (G13A) Mouse Monoclonal Antibody
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TD-261911
TD-261912
  • Catalog： TD-26191
  • Clonality： mAb
  • Application： ELISA, WB, IHC
  • Synonyms： Ras(G13A) 
  • Specification：
    100ul
  • Prices：
    ¥4800
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Anti-HRAS (G13A) Mouse Monoclonal Antibody

Cat. #:  TD-26191
Gene Symbol:  H-Ras; K-Ras; N-Ras
Description:   Anti-HRAS(G13A) Mouse Monoclonal Antibody
Background:   Belongs to the small GTPase superfamily. Ras family binds GDP/GTP and possess intrinsic GTPase activity, alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).Located in cell membrane, lipid-anchor, cytoplasmic side and Golgi apparatus membrane.
Immunogen:   A synthetic peptide from the internal region of HRas which includes the mutation of G13A, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:    ELISA: 1:1000-1:5000 、WB: 1:500-1:1000 、IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes G13A mutant, but not wild type H-RAS of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot: 

TD-261911.jpg
 

Immunofluorescence:   

TD-261912.jpg
 

Western blot analysis of recombinant HRAS(G13A) and wild type proteins. Purified His-tagged HRAS(G13A) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-HRAS(G13A) monoclonal antibody (Cat. #TD-26191).    

 

 

 

 

 

 

 

 

 

Immunofluorescence of cells expressing Ras proteins with Anti-HRAS(G13A) antibody. HEK293T cells were transfected with pCDNA3-GFP-HRas (WT) plasmid (left column) or pCDNA3-GFP-HRAS(G13A) plasmid (right column), then fixed and stained with Anti-HRAS(G13A) monoclonal antibody (Cat. #TD-26191).