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Point Mutation Antibody
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Anti-PIK3CA (E542Q) Mouse Monoclonal Antibody
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TD-262101
TD-262102
  • Catalog： TD-26210
  • Clonality： mAb
  • Application： ELISA, WB, IHC
  • Synonyms： PIK3CA(E542Q)
  • Specification：
    100ul
  • Prices：
    ¥4800
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Anti-PIK3CA (E542Q) Mouse Monoclonal Antibody

 

Cat. #:  TD-26210
Size:  100 µL
Gene Symbol:  PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
Description:   Anti-PIK3CA(E542Q) Mouse Monoclonal Antibody
Background:   Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
Immunogen:   A synthetic peptide from the internal region of PIK3CA which includes the mutation of E542Q, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes E542Q mutant, but not wild type PIK3CA of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Western blot: 

TD-262101.jpg

 
 Western blot analysis of recombinant PIK3CA(E542Q) and wild type proteins. Purified His-tagged PIK3CA(E542Q) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-PIK3CA(E542Q) monoclonal antibody (Cat. #TD-26210).  
Immunofluorescence: 

 

 Immunofluorescence of cells expressing PIK3CA proteins with Anti-PIK3CA(E542Q) antibody. HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA(E542Q) plasmid (right column), then fixed and stained with Anti-PIK3CA(E542Q) monoclonal antibody (Cat. #TD-26210).