All Products
  • Antibody
  • Kit
  • Peptide
  • Probe
  • Protein
  • Biochemical Reagent
  • Tissue Microarray
CH
ASGR1/ TL1A/ RSV/ TREM2/ Monkeypox virus/ BG4/ A29L/ IdeZ/ IdeS/ CD47/ claudin 18.2/ H3L/ SARS/ DNA/
Point Mutation Antibody
Path：Home > Product > Antibody > Point Mutation Antibody
Product
Product
Anti-PIK3CA (D549N) Mouse Monoclonal Antibody
All
TD-262151
TD-262152
  • Catalog： TD-26215
  • Clonality： mAb
  • Application： ELISA, WB, IHC
  • Synonyms： PIK3CA(D549N)
  • Specification：
    100ul
  • Prices：
    ¥4800
Customized service consulting
Information
FAQ
Experimental program
Related Products
Bibliography

Anti-PIK3CA (D549N) Mouse Monoclonal Antibody

 

Cat. #:  TD-26215
Size:  100 µL
Gene Symbol:  PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
Description:   Anti-PIK3CA(D549N) Mouse Monoclonal Antibody
Background:   Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
Immunogen:   A synthetic peptide from the internal region of PIK3CA which includes the mutation of D549N, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100
Concentration:   0.5 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes D549N mutant, but not wild type PIK3CA of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot: 

TD-262151.jpg
 
 Western blot analysis of recombinant PIK3CA(D549N) and wild type proteins. Purified His-tagged PIK3CA(D549N) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-PIK3CA(D549N) monoclonal antibody (Cat. #TD-26215).
Immunofluorescence: 

TD-262152.jpg

 
 Immunofluorescence of cells expressing PIK3CA proteins with Anti-PIK3CA(D549N) antibody. HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA(D549N) plasmid (right column), then fixed and stained with Anti-PIK3CA(D549N) monoclonal antibody (Cat. #TD-26215).