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Point Mutation Antibodies
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Anti-ALK (C1156Y) Mouse Monoclonal Antibody
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TD-262261
TD-262262
  • Catalog： TD-26226
  • Clonality： mAb
  • Application： ELISA, WB, IF, IHC
  • Synonyms： ALK(C1156Y)
  • Specification：
    100ul
  • Prices：
    ¥4800
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Anti-ALK (C1156Y) Mouse Monoclonal Antibody

 

Cat. #:   TD-26226
Gene Symbol:  ALK, CD246, NBLST3
Description:   Anti-ALK(C1156Y) Mouse Monoclonal Antibody
Background:   Anaplastic lymphoma kinase (ALK) also known as CD246 (cluster of differentiation 246) is a tyrosine kinase receptor that plays an important role in the development of the brain and nervous systems. Deficiencies in ALK, which most commonly as a fusion with other genes, are indicated in cancers.
Immunogen:   A synthetic peptide from the internal region of ALK which includes the mutation of C1156Y, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   ELISA: 1:1000-1:5000、WB: 1:500-1:2000、IF: 1:50-1:100、IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes C1156Y mutant, but not wild type ALK of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

TD-262261.webp
 Western blot analysis of recombinant ALK(C1156Y) and wild type proteins. Purified His-tagged ALK(C1156Y) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-ALK(C1156Y) monoclonal antibody (Cat. #TD-26226).

Immunofluorescence:  

TD-262262.webp

Immunofluorescence of cells expressing ALK proteins with Anti-ALK(C1156Y) antibody.

HEK293T cells were transfected with pCDNA3-GFP-ALK (WT) plasmid (left column) or pCDNA3-GFP-ALK(C1156Y) plasmid (right column), then fixed and stained with Anti-ALK(C1156Y) monoclonal antibody (Cat. #TD-26226).