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Point Mutation Antibodies
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Anti-EML4-ALK Mouse Monoclonal Antibody
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TD-262421
TD-262422
  • Catalog： TD-26242
  • Clonality： mAb
  • Application： ELISA, WB, IHC
  • Synonyms： EML4-ALK 
  • Specification：
    100ul
  • Prices：
    ¥4800
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Anti-EML4-ALK Mouse Monoclonal Antibody

Catalog Number:  TD-26242
Gene Symbol:  EML4-ALK
Description:   Anti EML4 ALK Mouse Monoclonal Antibody
Background:   EML4-ALK is a fusion-type protein kinase that can be found in many tumors, such as anaplastic large cell lymphoma, inflammatory myofibroblastic tumor, neuroblastoma and NSCLC, and so on. It is generated as a result of a small inversion within the short arm of human chromosome 2. EML4-ALK protein often promotes and maintains the malignant behavior of the cancer cells by activating the MAPK, PI3K/AKT and JAK/STAT3 pathways.
Immunogen:   A synthetic peptide from the internal region of EML4-ALK of human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   ELISA: 1:1000-1:2000 、WB: 1:500-1:1000 、IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes T41A mutant, but not wild-type EML4-ALK of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
Western blot:  
TD-262421.png
 

Western blot analysis of recombinant EML4 ALK and wildtype proteins. Purified His-tagged EML4-ALK protein (lane 3), corresponding wild-type EML4 protein (lane1) and wild-type ALK protein (lane2) were blotted with Anti EML4 ALK monoclonal antibody (Cat. #TD-26242).
Immunofluorescence:  
TD-262422.png
 
 Immunofluorescence of cells expressing EML4-ALK proteins with Anti EML4 ALK antibody. HEK293T cells were transfected with pCDNA3-GFP-EML4 (WT) plasmid (left column) or pCDNA3-GFP ALK(WT) plasmid (middle column) or pCDNA3-GFP-EML4-ALK plasmid (right column), then fixed and stained with Anti EML4 ALK monoclonal antibody (Cat. #TD-26242).