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Point Mutation Antibody
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Anti-EGFR (L747-P753) S Mouse Monoclonal Antibody
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TD-262871
TD-262872
  • Catalog： TD-26287
  • Clonality： mAb
  • Application： ELISA, WB, IF, IHC
  • Synonyms： EGFR (L747-P753)S
  • Specification：
    100ul
  • Prices：
    ¥4800
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Anti-EGFR (L747-P753) S Mouse Monoclonal Antibody

 

Cat. #:   TD-26287
Gene Symbol:  EGFR/HER1/ERBB1
Description:   Anti-EGFR(L747-P753)S Mouse Monoclonal Antibody
Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of (L747-P753) S, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR(L747-P753)S, but not wild type EGFR protein from vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
  Western blot:  
TD-262871.jpg
    

Western blot analysis of recombinant EGFR(L747-P753) S and wild type proteins.

Purified His-tagged EGFR(L747-P753)S protein (702-987) and wild type protein (702-987) were blotted with anti- EGFR(L747-P753)S mouse antibody (Cat. #TD-26287).

Immunofluorescence:  

TD-262872.jpg

Immunofluorescence of cells expressing EGFR proteins with anti-EGFR(L747-P753)S antibody.

HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(L747-P753)S plasmid (right column), then fixed and stained with anti- EGFR(L747-P753)S monoclonal antibody (Cat. #TD-26287).