CH
CD Antibodies
CD322
All
ELISA
WB
IF
FCM
  • CatalogTD-31590
  • ClonalitymAb
  • Application WB, IF, FCM
  • SynonymsJAM2; JAMB; JAM-B; VEJAM; PRO245; VE-JAM; C21orf43
  • Specification
    100ul 50ul
  • Prices
    ¥2180
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Mouse Monoclonal Antibody to CD322


Description
This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene.
 
Specification
Aliases JAM2; JAMB; JAM-B; VEJAM; PRO245; VE-JAM; C21orf43
Entrez GeneID 58494
Swissprot P57087
clone 7E4C5
WB Predicted band size 33.2kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human CD322 (AA: extra 29-238) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
IF 1/100
FCM 1/200 - 1/400
ELISA 1/10000
Product Image
TD-315901.jpg
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
 
 
TD-315902.jpg
Western blot analysis using CD322 mouse mAb against NIH/3T3 (1), Ramos (2), and HepG2 (3) cell lysate.
 

 

 
TD-315904.jpg
Immunofluorescence analysis of Hela cells using CD322 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
TD-315903.jpg
Flow cytometric analysis of HL-60 cells using CD322 mouse mAb (green) and negative control (red).