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G Protein Monoclonal Antibodies
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Anti-Gɑi Mouse Monoclonal Antibody
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  • Catalog： TD-26003
  • Clonality： 单克隆
  • Application： ELISA, WB, IHC
  • Synonyms：
  • Specification：
    100μl
  • Prices：
    ¥1600
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Anti- Gαi Mouse Monoclonal Antibody

Catalog Number:  TD-26003
Gene Symbol:  Gnai
Description:   Anti Gαi Mouse Monoclonal Antibody
Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gai represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gai proteins are being explored.
Immunogen:   Recombinant full length protein of Gαi1
Applications:   ELISA, WB, IHC
Recommended Dilutions:   ELISA 1:1000-1:5000,WB 1:1000-1:2000,IHC 1:100-1:200
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Gαi antibody recognizes Gαi1, Gαi2, and Gαi3 of vertebrates
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  

TD-26003.png

 

 Western blot analysis of Gi proteins. Gαi1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blots. The monoclonal anti-Gαi antibody was pre-incubated without (lane 1) or with (lane 2) the antigen protein (Gαi1) to show the specificity.