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Mouse β Amyloid （1-40）ELISA KIT
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  • Catalog： TD-BK901
  • Reactivity： Mouse
  • Sample： 血清、血浆、组织匀浆及相关液体
  • size：
    96T
  • Price：
    ¥询价
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Mouse Amyloid Beta 1–40 (Aβ 1–40) ELISA Kit

Statement: Please read the Mouse Aβ 1-40 Kit Instructions carefully before use. This kit is for research use only and must not be used for clinical diagnosis.

Cat. No.

TD-BK901

Size

96Test

Itended Use

 For the in vitro quantitative detection of Amyloid Beta 1–40 (Aβ 1–40) levels in serum, plasma, tissue homogenates, and related liquid samples.

Principle

 This kit utilizes a double-antibody sandwich Enzyme-Linked Immunosorbent Assay (ELISA). Samples, standards, and biotinylated detection antibodies are added sequentially to microwells pre-coated with mouse Aβ 1-40 capture antibodies. After incubation and thorough washing, TMB substrate is added for color development. Under the catalysis of peroxidase, TMB turns blue and eventually converts to yellow upon the addition of acid. The intensity of the color is positively correlated with the concentration of mouse Aβ 1-40 in the sample. Absorbance (OD value) is measured at a wavelength of 450 nm using a microplate reader to calculate the sample concentration.

Kit Components

Component

Configuration

Storage
Pre-Coated Microplate

12 wells × 8 strips

2-8℃
Standard

1×0.1 mL

-80℃
Sample Diluent

1×30 mL

2-8℃
Detection Antibody

1×0.1 mL

-80℃
Secondary Antibody

1×0.01 mL

-80℃
10×Wash Buffer

1×30 mL

2-8℃
TMB Substrate

1×18 mL

2-8℃
Stop Solution

1×8 mL

2-8℃
Instruction Manual

1 copy

 

 

Detection Range

 1.56 ng/mL – 100 ng/mL
Sensitivity Minimum detectable concentration is 400 pg/mL

Specificity

 No cross-reactivity with other soluble structural analogues

Reproducibility

 Intra-assay CV < 10%; Inter-assay CV < 15%

expiration

 6 months