Product Name |
Anti-6X His Tag antibody |
Cat.# |
TD-BYX-0013 |
Package |
25 μl/100 μl/200 μl |
Storage |
PBS with 0.1% sodium azide and 50% glycerol, pH 7.2 |
Product Imformation |
Description |
Anti-6×His Tag mouse monoclonal antibody |
Applications |
WB, IF, IP |
Immunogen |
Synthetic peptide conjugated with KLH |
Reactivity |
Fusion proteins containing the His Tag |
Host species |
Mouse |
Ig class |
IgG1 |
Purity |
Protein A/G Affinity Purification |
Target information |
Symbol |
6X His tag |
Full name |
HHHHHH |
|
|
Target Background |
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. |
Western blotting |
Predicted band size |
50 kDa |
Positive control |
Fusion proteins containing the His Tag |
Recommended dilution |
1000-5000 |
Product Image |
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