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Active Antibody
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Anti Active RhoA Mouse Monoclonal Antibody
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TD-26904
  • Catalog： TD-26904
  • Clonality： 单克隆
  • Application： IP, IHC
  • Synonyms： Active RhoA-GTP
  • Specification：
    30ul
  • Prices：
    ¥4800
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RhoA-GTP

 

Cat. #:   TD-26904
Size:  30 µL
Gene Symbol:  RHOA
Description:   Anti-RhoA-GTP Mouse Monoclonal Antibody
Background:   Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction.
Immunogen:   Recombinant full length protein of active RhoA
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG2b
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-RhoA monoclonal antibody recognizes active RhoA from vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western Blot:  
image.png		 IP/WB Analysis of Active RhoA Protein. Purified recombinant RhoA proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-RhoA-GTP monoclonal antibody (Cat. # TD-26904). After SDS/PAGE, the membrane filter was probed with anti-RhoA mouse monoclonal antibody.    
  • 推荐稀释比
    IP: 1 µg for 1~2 mg total cellular proteins; IHC: 1:50-1:250
  • 反应物种
    Mouse
  • 同种型
    IgG2b
  • 产品形式
    Liquid
  • 纯化方式
    Purified from ascites
  • 存储缓冲液
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol