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Active Antibody
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Anti Active Gao Mouse Monoclonal Antibody
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IP
  • Catalog： TD-26907
  • Clonality： 单克隆
  • Application： IP, IHC, IF
  • Synonyms： Active Gαo-GTP
  • Specification：
    30ul
  • Prices：
    ¥4800
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Gαo-GTP

 

 

Cat. #:   TD-26907
Size:  30 µL
Gene Symbol:  Gnao
Description:   Anti  Gαo-GTP Mouse Monoclonal Antibody
Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαo represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored.
Immunogen:   Recombinant full length protein of active Gαo
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG1
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active Gαo antibody recognizes active Gαo of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:  
 Gαo Activation Assay.
Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαo monoclonal antibody (Cat. No. TD-26907). Immunoblot was with an anti-Gαopolyclonal antibody .   
  • 推荐稀释比
    IP: 1 µg for 1~2 mg total cellular proteins; IHC,IF: 1:50-1:250
  • 反应物种
    Mouse
  • 同种型
    IgG1
  • 产品形式
    Liquid
  • 纯化方式
    Purified from ascites
  • 存储缓冲液
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol