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Active Antibody
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Anti Active Ras Mouse Monoclonal Antibody
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TD-26909
  • Catalog： TD-26909
  • Clonality： 单克隆
  • Application： IP, IHC, IF
  • Synonyms： Active Ras-GTP
  • Specification：
    30ul
  • Prices：
    ¥4800
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Ras-GTP

 

Cat. #:   TD-26909
Size:  30 µL
Gene Symbol:  RAS
Description:   Anti-Ras-GTP Mouse Monoclonal Antibody
Background:   Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
Immunogen:   Recombinant full length protein of active H-Ras
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Ras-GTP monoclonal antibody recognizes active H-Ras, N-Ras, and K-Ras from vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation/Western blot:  

TD-26909_Ras-GTP.jpg
 
 IP/WB Analysis of Active Ras Protein.Purified recombinant Ras proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-Ras-GTP monoclonal antibody (Cat. # TD-26909). After SDS/PAGE, the membrane filter was probed with anti-Ras mouse monoclonal antibody.  
  • 推荐稀释比
    IP: 1 µg for 1~2 mg total cellular proteins; IHC,IF: 1:50-1:250
  • 反应物种
    Mouse
  • 同种型
    IgG
  • 产品形式
    Liquid
  • 纯化方式
    Purified from ascites
  • 存储缓冲液
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol