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Active Antibody
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Anti Active Gaz Mouse Monoclonal Antibody
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IP
  • Catalog： TD-26908
  • Clonality： 单克隆
  • Application： IP, IHC, IF
  • Synonyms： Active Gαz-GTP
  • Specification：
    30ul
  • Prices：
    ¥4800
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Gαz-GTP

 

Cat. #:   TD-26908
Size:  30 µL
Gene Symbol:  Gnaz
Description:   Anti-Gαz-GTP Mouse Monoclonal Antibody
Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαz represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαz proteins are being explored.
Immunogen:   Recombinant full length protein of active Gαz
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Gαz-GTP monoclonal antibody recognizes active Gαz of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:  
 Gαz activation assay. Purified Gαz proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-Gαz-GTP monoclonal antibody (Cat. No. TD-26908). Immunoblot was with an anti-Gαz polyclonal antibody.  
  • 推荐稀释比
    IP: 1 µg for 1~2 mg total cellular proteins; IHC,IF: 1:50-1:250
  • 反应物种
    Mouse
  • 同种型
    IgG
  • 产品形式
    Liquid
  • 纯化方式
    Purified from ascites
  • 存储缓冲液
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol